Poster abstracts
Poster number 51 submitted by Archie Bhullar
Small RNAs Physiological Diffusion Dynamics in the Vasculature
Archie Bhullar (Biophysics ), Xin Li (Pharmacy, Ohio State University), Daniel Binzel (Pharmacy, Ohio State University), Peixuan Guo (Pharmacy, Biophysics, Ohio State University)
Abstract:
Using Oligonucleotides as biopolymer material in the medical application of nanostructures for drug delivery, as a targeting aptamer, or as a drug such as miRNA, siRNA, or mRNA, requires unique biophysical characterization in vivo for full utilization in the medical field. Among these unique biophysical elements, is the characterization of chemically modified small RNAs trans endothelial vasculature diffusional flux. The diffusional flux of small RNAs in several areas of the vasculature including the ectopic tumor vasculature and glomerular membrane in the kidneys are clinically relevant. Here I provide a preliminary rationale and methodology for the investigation of RNA trans-vasculature flux dependent on vasculature composition, pressure, and temperature. Furthermore, I propose the rationale for the design of a nanoparticle carrier with a thermodynamically unstable core and pseudoknot helix arms for flexible stoichiometry when coupled with the dynamic nature of RNA structure in vivo.
Keywords: RNA, Diffusion