Poster abstracts
Poster number 38 submitted by Gregory Jameson
A Prior Knowledge Non-Uniform Sampling Method to Achieve Minimal Sampling in Multidimensional NMR
Gregory Jameson (Biophysics Graduate Program, The Ohio State University ), Alexandar L. Hansen (Campus Chemical Instrument Center, The Ohio State University ), Dawei Li (Campus Chemical Instrument Center, The Ohio State University ), Rafael Brschweiler (Department of Chemistry and Biochemistry, Campus Chemical Instrument Center, Department of Biological Chemistry and Pharmacology, The Ohio State University)
Abstract:
NMR is a powerful tool for characterizing proteins, capable of determining structural and dynamic information in the picosecond to second time scale at atomic resolution. One of the dynamics methods available, chemical exchange saturation transfer (CEST), is able to inform on domain motion and slow folding/unfolding dynamics, including exchange with an excited state with less than 1% population. However, for a given residue, the occurrence of CEST to yield a positive result is quite rare, and the experiment can take several days on a NMR spectrometer. In order to substantially reduce the NMR time needed and allow for samples that become unstable in shorter timespans, we present a novel non-uniform sampling method for use on CEST and similar dynamics experiments. We also use this new method to systematically analyze choice of NMR evolution time increments taken in non-uniform sampling experiments at a scale never before done, which has significant impact on the wider field of non-uniform sampling.
Keywords: NMR, protein, dynamics