Poster abstracts

Poster number 20 submitted by Callie Lovejoy

Role of Hat1 during DNA replication coupled chromatin assembly

Callie M. Lovejoy (MCDB)

Abstract:
Efficient and correct cellular function is reliant on a stable genome that maintains its integrity through many cell divisions. Genome corruption is responsible for the development of many disease states, pathogen and cancer drug resistance, and decreased cellular, and thus organismal, fitness. Histone acetyltransferase 1 (Hat1) is responsible for acetylating Lysines 5 and 12 on newly synthesized Histone H4 during DNA replication coupled chromatin assembly. Hat1 -/- mouse embryonic fibroblasts exhibit genome instability such as chromosome breaks and fusions. Previous research has shown that loss of Hat1 alters the composition of proteins on nascent chromatin. This study identified Centrosomal Protein of 162 kDa (CEP162) as present on nascent chromatin of wild type cells but highly depleted on Hat1 -/- nascent chromatin. CEP162 is a centrosomal protein involved in cilia formation and chromosome segregation but has no known chromatin function. The mechanisms of Hat1 and CEP162 in maintaining genome stability during DNA replication remain unknown. In this project, we want to ask what the biological function of CEP162 is in relation to nascent chromatin and why the presence of CEP162 on nascent chromatin is Hat1 dependent.

References:
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Keywords: Hat1, genome stability, chromatin assembly