Poster abstracts

Poster number 82 submitted by SungSuk Suh

A MicroRNAs control expression of critical proteins for multiple myeloma development

Sung-Suk Suh (CCC), Flavia Pichiorri (CCC)

Abstract:
Progress in understanding biology and genetic, and in advancing therapy of Multiple myeloma(MM), a plasma cell malignancy, has been slow. The discovery of microRNAs(miRNA), a class of small non-coding RNAs targeting multiple mRNAs, has revealed a new level of gene expression regulation. To determine whether miRNAs play a role in the malignant transformation of plasma cells (PCs), we compared the miRNA gene expression of MM plasma cells to that in normal cells, especially, mir-25, 32, and 92. We found significant over-expression of these mirRNAs compared with normal PCs. We had selected FBXW7 (F-box/WD repeat protein 7) ,which is a member of the F-box protein family The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), as target gene by using the specific software program. To determine whether FBXW7 is real target gene, we made construct including 3’-untranslated region of FBXW7 subcloned at 3’-UTR of luciferase reporter gene. We had observed that the luciferase activity was significantly decreased after co-transfection with mirRNAs and luciferase plasmid containing 3’-UTR of FBXW7 compared to negative control. In further studies, we will characterize the relationship between mirRNAs and their target gene, FBXW7, in detail.

Keywords: MirRNA, FBXW7, multiple myeloma