Poster abstracts

Poster number 52 submitted by Myron Ignatius

colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis.

Holly E. Moose (Center for Molecular and Human Genetics, Columbus Childrens Research Institute), Heithem M. El-Hodiri (Center for Molecular and Human Genetics, Columbus Childrens Research Institute), Paul D. Henion (MCDB, Center for molecular neurobiology, Department of Neuroscience)

Abstract:
Neural crest-derived pigment cell development has been used extensively to study cell fate specification, migration, proliferation, survival and differentiation. Many of the genes and regulatory mechanisms required for pigment cell development are conserved across vertebrates. The zebrafish mutant colgate (col) /histone deacetylase1 (hdac1) has reduced numbers, delayed differentiation and decreased migration of neural crest-derived melanophores and their precursors. In hdac1col mutants, normal numbers of premigratory neural crest cells are induced. Later, while there is only a slight reduction in the number of neural crest cells in hdac1col mutants, there is a severe reduction in the number of mitfa-positive melanoblasts suggesting that hdac1 is required for melanoblast specification. Concomitantly there is a significant increase in and prolonged expression of foxd3 in neural crest cells in hdac1col mutants. We found that partially reducing Foxd3 expression in hdac1col mutants rescues mitfa expression and the melanophore defects in hdac1col mutants. Further, we demonstrate the ability of Foxd3 to physically interact at the mitfa promoter. Because mitfa is required for melanoblast specification and development, our results suggest that hdac1 is normally required to suppress neural crest foxd3 expression thus de-repressing mitfa resulting in melanogenesis by a subset of neural crest-derived cells.
Keywords: neural crest, melanophore, histone deacetylase1, foxd3, mitfa; c-kit, zebrafish

Keywords: neural crest, melanophore, histone deacetylase1, foxd3, mitf, zebrafish