Poster abstracts

Poster number 11 submitted by Jeffrey Voorhees

The Effects of Zinc on the Interaction Between Lactogenic Hormones and the Human Prolactin Receptor

Jeffrey L. Voorhees (The Ohio State Biochemistry Program and Department of Veterinary Biosciences, The Ohio State University), Charles L. Brooks (The Ohio State Biochemistry Program and Department of Veterinary Biosciences, The Ohio State University)

Abstract:
Human prolactin (hPRL), growth hormone (hGH), and placental lactogen (hPL) are cytokine family members that are capable of binding and activating the human prolactin receptor (hPRLr). Structural and biological work have demonstrated that zinc is directly involved in hormone-receptor complex formation. However, little work has been done to assess the importance of zinc concentration in experimental conditions. Concentrations of circulating zinc vary significantly throughout the life of an individual and the role and activity of lactogenic hormones similarly vary. Previous examinations of the role of zinc in hormone-receptor binding have focused on demonstrating binding and identifying factors that mitigate it. This work utilizes surface plasmon resonance (SPR), novel mathematical modeling, FRET, and biological assays to illuminate previous work by directly comparing the effects of zinc on the particular kinetic, conformational, and biological changes in the interaction of these hormones with hPRLr.

References:
Arslan, P., Di Virigilio, F., Beltrame, M., Tsien, R.Y., Pozzan, T. (1985) J Biol Chem. 260 (5), 2719-2727.
Norbert W. Tietz. Textbook of Clinical Chemistry. Philaadelphia: W.B. Saunders Company, 1986.
Cunningham B.C., Bass S., Fuh G., Wells J.A. (1990) Science. 250 (4988),1709-12.
Cunningham, B.C., Wells, J. A. (1991) Proc. Natl. Acad. Sci. 88, 3407-3411.
Dannies, P.S. (2001) Trends in Endocrinology & Metabolism. 12 (10):427-8.
Sivaprasad, U., Canfield, J.M., and Brooks, C.L. (2004) Biochemistry. 43, 13755-13765
Somers, W., Ultsch, M., De Vos, A.M., Kossiakoff, A. (1994) Nature. 372(6505):478-81.

Keywords: cytokine, SPR, zinc