Poster abstracts
Poster number 7 submitted by Shekaraiah Devari
Design and synthesis of potent DNA/RNA binding shortest di, tri and cyclic bifacial peptide nucleic acids (bPNAs)
Dr. Shekaraiah Devari (The Ohio State University), Prof. Dennis Bong (The Ohio State University)
Abstract:
Design and synthesis of a small library of alpha/beta isopeptide-nucleic acid has been untangle to explore the influence of various backbone carbon chain length and spacers in between the two monomers on the hybridization properties of the DNA or RNA. The screening of melamine (M) conjugated various amino acid derivatives, Diaminopropionic acid (Dp), Diaminobutyric acid (Db), ornithine (O) and lysine (K), have been performed. The hydrophobic amino acid D/L-alanine, D/L-proline and glycine has been introduced as a spacer. Melting temperature (Tm) analysis suggests that dipeptide having alpha-K2M bPNA exhibited the significant binding compared to various bPNAs having the spacer amino acids, i.e D or L-alanine, D or L-proline and glycine with both T/U rich DNA/RNAs. Furthermore, the mixed monomers containing bPNAs fail to exhibit the suitable binding T/U rich DNAs/RNAs. Interestingly, the cyclic bPNA having 4C linker K2M diketopiperazine (DKP) determines the greater binding against T/U rich DNAs/RNAs with respect to the linear di and tri-peptides as well as other DKPs (diketopiperazine's) with 1-3C linkers according to the thermal melting analysis (Tm).
References:
1) S. Miao, Y. Liang, I. Marathe, J. Mao, C. DeSantis, and D. Bong, J. Am. Chem. Soc. 2019, 141, 9365−9372.
2) Y. Liang, S. Miao, J. Mao, C. DeSantis, and D. Bong, Biochemistry 2020, 59, 2410−2418.
Keywords: RNA and DNA binding , bPNAs, Thermal melting