Poster abstracts
Poster number 8 submitted by Shekaraiah Devari
Design and Synthesis of DNA/RNA Targeted Novel α-PNAs Based on Amino Side Chains
Shekaraiah Devari (Chemistry & Biochemistry), Debmalya Bhunia (Chemistry & Biochemistry)
Abstract:
Most recently, DNA/RNA targeted molecules have achieved significant attention around the globe due to huge implications in the field of drug discovery. Among them, α-PNAs (Peptide-nucleic acid) play potential role as they target DNA/RNA through base pairing recognition and significant binding efficacy.1 Herein, we have designed and synthesized a library novel peptide cognates (α-PNA) consisted with Melamine (M) and various nucleobases [cytosine (C), Uracil (U), Adenine (A), and Guanine (G)] having amino side chains. Most interestingly, our newly synthesized α-PNAs exhibit significant thermal denaturation (Tm) with targeted ssDNA with higher specificity. These DNA/RNA mimicking peptides may target the non-coding RNA (MALAT1), extremely overexpressed in various cancer cells (lung, pancreatic, cervical etc).2 Our focus is to knockdown the MALAT1 gene, ultimately reduce the tumor growth and metastasis in pre-clinical studies.
Keywords: DNARNA, MALAT1, Peptide Nuclic Acids