Putative trypanosome guide RNAs in mitochondria amd in purified MRB complexes

This page provides access to the aligned sequencing data for different libraries of putative guide RNAs in Trypanosoma brucei brucei, Lister strain 427 (procyclic form 29:13) and Eatro164 stock (procyclic form). The experimental details are described in the publications:

Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition.
Madina BR, Kumar V, Metz R, Mooers BH, Bundschuh R, Cruz-Reyes J (2014)
RNA 20:1142-52. doi: 10.1261/rna.044495.114
BioSample accession SAMN02795843.

and

The insect-phase gRNA transcriptome in Trypanosoma brucei.
Koslowsky D, Sun Y, Hindenach J, Theisen T, Lucas J. (2014)
Nucleic Acids Res. 42:1873-86. doi: 10.1093/nar/gkt973
BioSample accession SAMN02204165.

Putative guide RNA alignments

• unenriched procyclic library (Eatro164 stock)
• unenriched procyclic library (Lister strain 429:29-13)
• 3010-MRB pulldown library (Lister strain 429:29-13)
• REH2-MRB pulldown library (Lister strain 429:29-13)

This material is based upon work supported by the National Science Foundation under Grant Numbers MCB-1122109 and DMR-1105458. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.