Poster abstracts
Poster number 24 submitted by Megan Allyn
Oxidative Stress Responsive Nanoparticles for Sustained Delivery of Proteins for Advancing Treatment of Age-Related Macular Degeneration
Megan M. Allyn (Department of Chemical and Biomolecular Engineering, the Ohio State University), Andre F. Palmer (Department of Chemical and Biomolecular Engineering, the Ohio State University), Katelyn E. Swindle-Reilly (Department of Chemical and Biomolecular Engineering, Department of Biomedical Engineering , Department of Opthamology and Visual Sciences, the Ohio State University)
Abstract:
Age-related macular degeneration (AMD) is a leading cause of blindness globally, with prevalence of the disease increasing with age. Oxidative stress from accumulated reactive oxygen species (ROS) and chronic inflammation propagate cellular and tissue damage, leading to lipid peroxidation, retinal pigment epithelium (RPE) dysfunction, and vision loss via loss of RPE and/or pathological angiogenesis. While previously untreatable, advancements in therapeutics provide disease management but at a high cost to patients, requiring frequent invasive and costly intravitreal injections for proper disease mitigation. Reduction in injection frequency is a main focus of posterior segment drug delivery and has shown great promise with the incorporation of biomaterials. This research investigates oxidative stress responsive polydopamine nanoparticles, loaded with an anti-inflammatory and antioxidant novel protein therapeutic, for sustained extended dosing, beyond what is achievable with current approved therapies. Nanoparticles were synthesized via self-polymerization and characterized by dynamic light scattering and ZetaPals analyzer, as well as explored for their therapeutic effectiveness in vitro. They provided antioxidant relief to primary porcine retinal cells as measured by DCFDA assay after co-treatment of oxidative challenge. Taken together, the protein therapeutic loaded polydopamine nanoparticles provide antioxidant protection against ocular degeneration.
Keywords: Opthalmology, Drug Delivery , Nanoparticles