Poster abstracts
Poster number 24 submitted by Caden Martin
Regulation of Constitutive Heterochromatin by Histone Acetyltransferase 1
Caden Martin (Department of Biological Chemistry and Pharmacology), Prabakaran Nagarajan (Department of Biological Chemistry and Pharmacology), Callie Lovejoy (Department of Biological Chemistry and Pharmacology), Mark Parthun (Department of Biological Chemistry and Pharmacology)
Abstract:
Cellular memory relies in large part on the ability of cells to reliably transmit the information contained in histone posttranslational modifications from parental to daughter DNA during S-phase. This inheritance may be regulated by histone acetyltransferase 1 (HAT1), a highly conserved protein that chaperones newly synthesized histones and di-acetylates all new histone H4 on lysines 5 and 12 prior to its deposition throughout the genome in S-phase. Based on recent results in our lab, we tested the hypothesis that HAT1 regulates the balance of heterochromatin and euchromatin across of the genome. Using a method of profiling histone modifications known as CUT&Tag, we show here that HAT1 loss results in depletion of activating marks such as H3K9ac, H3K27ac, H3K4me1, and H4K5ac within large heterochromatic domains called HAT1-dependent accessibility domains (HADs), while increasing the levels of constitutive heterochromatin hallmarks such as H3K9me3. Interestingly, the regions regulated by HAT1 strongly correspond with regions associated with the nuclear lamina. These results suggest that HAT1 is essential for proper maintenance of constitutive heterochromatin at the nuclear periphery.
Keywords: HAT1, Epigenetics, Epigenetics