Poster abstracts
Poster number 65 submitted by Debasmita Mukherjee
Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
Debasmita Mukherjee (James Comprehensive Cancer Center, The Ohio State University, Columbus, USA), Mallory J. DiVincenzo (James Comprehensive Cancer Center, The Ohio State University, Columbus, USA; Department of Veterinary Biosciences, The Ohio State University, Columbus, USA), Molly Torok, Fouad Choueiry, Rahul J. Kumar (James Comprehensive Cancer Center, The Ohio State University, Columbus, USA), Jessica L. Cooperstone (Department of Food Science and Technology, The Ohio State University, Columbus, USA; Departments of Horticulture and Crop Science, The Ohio State University, Columbus, OH, 43210, USA), Phil A. Hart (Division of Gastroenterology, Hepatology, and Nutrition, The Ohio State University Wexner Medical Center), Thomas A. Mace (James Comprehensive Cancer Center, The Ohio State University, Columbus, USA; Division of Gastroenterology, Hepatology, and Nutrition, The Ohio State University Wexner Medical Center)
Abstract:
Chronic pancreatitis (CP) is a fibro-inflammatory syndrome which develops upon persistent pathological responses to parenchymal injury. Novel dietary interventions that lessen inflammation in this disease could significantly improve quality of life. Complex dietary foods like soy and tomatoes contain metabolites with anti-inflammatory effects. Data from our group reports that bioactive agents in soy and tomatoes can reduce pro-inflammatory cytokines and suppressive immune populations. Additionally, our team has developed a novel soy-tomato juice currently being studied in healthy individuals with no toxicities, and good compliance and bioavailability. Therefore, we hypothesize that administration of a soy-tomato enriched diet can reduce inflammation and severity of CP. C57BL/6 mice were injected intraperitoneally with 50 μg/kg caerulein (7 hourly injections twice a week) for 6 weeks to induce CP. After 4 weeks of caerulein injections, mice were administered a control or a soy-tomato enriched diet for 2 weeks. Disease severity was measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis. Serum lipase and amylase levels were analyzed at the end of the study. Inflammatory factors in the serum and pancreas, and immune populations in the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respectively. Infra-red sensing of mice was used to monitor spontaneous activity and distress of mice. Mice fed a soy-tomato enriched diet had a significantly reduced level of inflammation and severity of CP compared to mice administered a control diet with restored serum lipase and amylase levels. Mice with CP fed a soy-tomato diet had a reduction in inflammatory factors (TNF-α, IL-1β, IL-5) and suppressive immune populations (MDSC) compared to control diet fed mice. Infra-red sensing to monitor spontaneous activity of mice showed that soy-tomato enriched diet improved total activity and overall health of mice with CP and CP mice on a control diet were determined to spend more time at rest. These pre-clinical results indicate that a soy-tomato enriched diet may be a novel treatment approach to reduce inflammation and pain in patients with CP.
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Keywords: Chronic Pancreatitis, Inflammation, Dietary intervention