Poster abstracts
Poster number 41 submitted by Ahmet Ata Akatay
Peripheral Regions of Clathrin-coated Plaques are Active Endocytic Sites
Ahmet Ata Akatay (Biophysics Program), Spencer P. Heidotting (Department of Physics), Nathan M. Willy (Department of Physics), Comert Kural (Department of Physics)
Abstract:
Clathrin-coated structures are the best characterized endocytic carriers, but the mechanism through which clathrin-coated plaques perform endocytic functions is still debated. We developed novel experimental and analytical approaches that allow us to dissect the mechanism of plaque growth and disassembly with high spatial and temporal resolution. We found that clathrin and its primary adaptor protein (AP2) are incorporated to the plaques at the peripheral regions. Patches of clathrin coats are internalized also from the peripheries. Our results show that the peripheral regions of plaques are active endocytic sites whereas the central regions are more stable.
References:
AA Akatay, SP. Heidotting, NM. Willy and C. Kural. “Peripheral Regions of Clathrin-coated Plaques are Active Endocytic Sites” submitted to Journal of Cell Science.
Keywords: light sheet microscopy, total internal reflection microscopy, clathrin mediated endocytosis