Poster abstracts

Poster number 77 submitted by Qiming Li

Multiple transcription start site clusters of mouse IL-1R1 gene

Qiming Li (Department of Oral Biology, Ohio State University, Columbus, Ohio 43210), Ling Zhu (Department of Oral Biology, Ohio State University, Columbus, Ohio 43210), Hao Zhang (Department of Oral Biology, Ohio State University, Columbus, Ohio 43210), Qun Chen (Department of Oral Biology, Ohio State University, Columbus, Ohio 43210), Ning Quan (Department of Oral Biology, Ohio State University, Columbus, Ohio 43210)

Abstract:
IL-1R1 is the only known functional receptor for IL-1 signaling. Transcription and expression of IL-1R1 on diverse cell types may mediate various IL-1 induced effects. The mechanism of IL-1R1 transcriptional regulation has not been fully studied. Identification of precise transcription start site (TSS) is important for transcriptional regulation study. In previous studies, the TSSs of mIL-1R1 were determined by either primer extension or 5’ RACE. However, neither primer extension nor 5’ RACE is able to distinguish full-length 5’ capped mRNA from truncated mRNA. In addition, mRNA from limited types of mouse tissues was used in previous studies. In current study, CapFishing kit, which specifically generates cDNA from 5’ cap intact mRNA, was used to determine the TSSs of mIL-1R1 in dentate gyrus, hypothalamus, liver and lung. Four clusters of mIL-1R1 TSSs were found. Three of them are in agreement with the previous study by our lab (using 5’RACE), although more than thirty bps of 5’ UTR region are further extended in two of the clusters compared with previous study. We have identified a novel fourth TSS cluster which resides downstream of classic start codon, indicating the existence of a novel internal promoter. Activation of this promoter is predicted to produce a truncated mIL-R1 protein.

Keywords: TSS, IL-1R1