Poster abstracts
Poster number 27 submitted by Jennifer Kist
HPLC fractionation and LC-MS/MS characterization of human tRNA species
Jennifer A. Kist (Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati), Cassandra Herbert (Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati), Patrick A Limbach (Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati)
Abstract:
Mass spectrometry - especially LC-MS/MS methods - are becoming increasingly important for the characterization of post-transcriptional modifications on ribonucleic acids (RNAs) including using LC-MS/MS to map modifications to specific locations within an RNA sequence. Researchers have shown that RNA Modification Mapping by LC-MS/MS is applicable to mixtures of RNAs (in particular, transfer RNAs - tRNAs) from bacterial and archaeal organisms. However, many organisms - especially eukaryotes - can transcribe hundreds or more individual tRNA sequences within the cell. Such mixtures are inherently difficult to accurately map modifications to particular sequences given current technologies. A goal of this work is to identify new approaches for RNA fractionation compatible with RNA modification mapping.
Keywords: tRNA, LC-MSMS, RP-HPLC