Poster abstracts
Poster number 53 submitted by Benjamin Warner
Roles of the leader-trailer helix and antitermination complex in biogenesis of the 30S ribosomal subunit
Benjamin Warner (Department of Microbiology, Center for RNA Biology), Ralf Bundschuh (Center for RNA Biology, Department of Physics, Department of Chemistry and Biochemistry, Division of Hematology), Kurt Fredrick (Department of Microbiology, The Ohio State University)
Abstract:
Ribosome biogenesis occurs co-transcriptionally and entails rRNA folding, r protein binding, rRNA processing, and rRNA modification. In most bacteria, the 16S, 23S, and 5S rRNAs are co-transcribed, often with one or more tRNAs. Transcription involves a modified RNA polymerase, called the antitermination complex, which forms in response to cis-acting elements (boxB, boxA, and boxC) in the nascent pre-rRNA. Sequences flanking the rRNAs are complementary and form long helices known as leader-trailer helices. Here, we employed an orthogonal translation system to interrogate the functional roles of these RNA elements in 30S subunit biogenesis in Escherichia coli. Mutations that disrupt the leader-trailer helix caused complete loss of translation activity, indicating that this helix is absolutely essential for active subunit formation in the cell. Mutations of boxA also reduced translation activity, but by only 2- to 3-fold, suggesting a smaller role for the antitermination complex. Similarly modest drops in activity were seen upon deletion of either or both of two leader helices, termed here hA and hB. Interestingly, subunits formed in the absence of these leader features exhibited defects in translational fidelity. These data suggest that the antitermination complex and precursor RNA elements help to ensure quality control during ribosome biogenesis.
Keywords: ribosome biogenesis, RNA structure, precursor RNA