Poster abstracts
Poster number 29 submitted by Yi-Meng Lo
bPNA probes for RNA-Protein interaction
Yi-Meng Lo (Chemistry)
Abstract:
Deciphering RNA-protein and RNA-RNA interactions are crucial for regulating the function of the RNA. Herein, we describe a novel RNA-centric method to label RNPs using bifacial peptide nucleic acids (bPNAs). The labeling method developed in our group takes advantage of the affinity of bPNA for U-rich internal loops (URILs) in structured RNA. These URILs can be installed in RNAs of interest, which enables binding with bPNA targeting of RNAs and RNPs. Herein, we describe how appropriately modified bPNAs can track intracellular RNPs with fluorogenic labels and direct the oxidation chemistry to RNPs and the RNA/DNA complex. In prior work, we have demonstrated that bPNA outfitted with fluorogenic dye (thiazole orange) and redox-active centers (iron-EDTA, or copper phenanthroline) can effectively label and oxidize RNPs, resulting in crosslinking with biotin-phenol. We describe further experiments herein that expand the scope of bPNA-hybrid directed labeling for tracking and spectroscopic characterization of RNPs as well as RNP proximity biotinylation.
Keywords: RNA-protein interaction, proximity labeling, bPNA