Poster abstracts
Poster number 23 submitted by Kai Jin
RNA micelles for the delivery of therapeutics and their properties
Kai Jin (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA), Hongran Yin (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA), Nasir Uddin (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA), Daniel Binzel (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA), Dan Shu (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA), Peixuan Guo (Center for RNA Nanobiotechnology and Nanomedicine, Division of Pharmaceutics and Pharmacology, College of Pharmacy, the Ohio State University, Columbus, OH 43210, USA)
Abstract:
The RNA micelles have shown advantages in cancer targeting and drug delivery, therefore showing The RNA micelles have shown advantages in cancer targeting and drug delivery, therefore showing their potential for becoming the next-generation cancer treatment platform. Herein, we conjugate cholesterol to the helical ends of pRNA's branched three-way junction (3WJ) motif to build RNA micelles. The resulting amphipathic RNA micelles consist of hydrophilic RNA heads and hydrophobic lipid tails linked covalently, which can spontaneously assemble micelles in aqueous solutions through hydrophobic interactions. By exploiting the branched structure of 3WJ, RNA micelles can combine various functional modules for different targeting and treatment purposes. As a proof-of-concept, paclitaxel is able to be loaded into RNA micelles, and the water solubility of paclitaxel is significantly improved. A 3WJ-Micelle structure is formed after the annealing process, and the mean size is 118.7 nm. The estimated critical micelle-forming concentrations ranged from 39 nM to 78 nM. Paclitaxel-loaded RNA micelles were taken up by tumor cells and inhibited tumor cell proliferation as confirmed by an MTT assay and a caspase-3-assay. Finally, paclitaxel-loaded RNA micelles can reach tumors in vivo without accumulating in healthy tissues and organs. In addition, there is little or no pro-inflammatory response. Thus, multivalency, tumor cell permeability, controlled assembly, low or no toxicity, and tumor-targeting properties make pRNA micelles a viable platform for potential drug delivery.
References:
1. Guo, P., The emerging field of RNA nanotechnology. Nat Nanotechnol 2010, 5 (12), 833-42.
2. Shu, Y.; Yin, H.; Rajabi, M.; Li, H.; Vieweger, M.; Guo, S.; Shu, D.; Guo, P., RNA-based micelles: A novel platform for paclitaxel loading and delivery. J Control Release 2018, 276, 17-29.
Keywords: RNA Nanotechnology, Drug Delivery, RNA micelle