Poster abstracts
Poster number 24 submitted by Yi-Meng Lo
bPNA probes for RNA-Protein interaction
Shiqin Miao (Chemistry & Biochemistry), Yi-Meng Lo (Chemistry & Biochemistry)
Abstract:
Deciphering RNA-protein and RNA-RNA interactions is crucial for regulating the function of the RNA. Herein, we describe a novel method to label RNPs using bifacial peptide nucleic acids. The labeling method developed in our group takes advantage of the affinity of bPNA for U-rich internal loops (URILs) in structured RNA. These URILs can be installed in RNAs of interest and bPNA can direct the chemistry of the RNA complex. In prior work, we have demonstrated that bPNA outfitted with iron-EDTA or copper phenanthroline can generate the reactive oxygen species under redox conditions, resulting in directed oxidative cleavage of proximal DNA sites. We describe experiments herein that expand the scope of bPNA-hybrid directed oxidation chemistry.
References:
Ramanathan, M.; Porter, D. F.; Khavari, P. A. Methods to Study RNA-Protein Interactions. Nat. Methods 2019, 16 (3), 225–234.
Zheng, X.; Cho, S.; Moon, H.; Loh, T. J.; Jang, H. N.; Shen, H. Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting. Methods Mol. Biol. 2016, 1421, 35–44.
Keywords: RNA-protein interaction, bPNA, URIL