Poster abstracts
Poster number 67 submitted by Congcong Xu
Development of RNA Nanoparticles with Controllable Size and Shape Using Planar Phi29 pRNA Three-Way Junction
Congcong Xu (College of Pharmacy/Division of Pharmaceutics and Pharmaceutical Chemistry; College of Medicine/Department of Physiology & Cell Biology/Dorothy M. Davis Heart and Lung Research Institute; The Ohio St), Daniel L. Jasinski (College of Pharmacy/Division of Pharmaceutics and Pharmaceutical Chemistry; College of Medicine/Department of Physiology & Cell Biology/Dorothy M. Davis Heart and Lung Research Institute; The Ohio St), Kaiming Zhang (Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, Texas), Wah Chiu (Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, Texas), Luda Shlyakhtenko (Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE), B. Mark Evers (3Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY)
Abstract:
Precise control over size of nanoparticles is attractive and critical for biomedical applications. Controllable assembly of nanometer-sized architecture leads to well-defined stoichiometry and programmable composition of nanoparticles. RNA is emerging as a unique material and building unit for nanoparticle assembly due to its simplicity characteristic in structure and diversity feature in function. Bacteriophage phi29 packaging RNA (pRNA) is a key component of the viral DNA-packaging motor. The pRNA forms a hexameric ring to gear one of the most powerful bionanomotors known to date. The three-way junction (3WJ) core of pRNA exhibits unusual thermodynamic stability under denaturing conditions. Herein we present the uniqueness of 3WJ and the controllable assembly of RNA nanoparticles with defined size derived from planar 3WJ.
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Keywords: RNA nanoparticles, Controllable size and shape, Three-way junction