Poster abstracts
Poster number 45 submitted by Callie Moore
Development of binding and stability assays of the anti-tumor Ab fragment 3E8 for high throughput cancer screening
Callie L. Moore (College of Arts and Sciences), Nicholas E. Long (Ohio State Biochemistry Program), Thomas J. Magliery (Ohio State Department of Chemistry and Biochemistry)
Abstract:
Tragedy falls upon more than 1,500 people a day when they lose their battle with cancer. Surgical resection of the cancerous tumor continues to be one of the most successful methods of cancer treatment. In order to remove cancerous tumors, the surgeon needs to have the ability to differentiate healthy from diseased tissue. Doctors use cancer imaging for this specific reason. In order to see the cancer, a tumor antigen, which is an antigenic substance produced within tumor cells, can be used as a marker. One of these tumor antigens is Tag72 which contains a unique sugar, Sialyl-Tn. The Magliery lab has engineered an antibody fragment, 3E8.scFv, that is able to specifically bind to this disaccharide. Using the diagnostics developed in this lab, it is our hope that surgeons will soon have a more proactive way to screen for cancer. There are still many tests needed to be performed in order to make sure this method is as safe and effective for the patients as possible. Our research is working towards learning more about the binding and stability properties of the antibody 3E8 in order to provide an effective way for surgeons to screen for cancer and safely remove the cancerous tumor. Specifically, my research involves the performance of immunohistochemistry on cancerous tissues positive for Tag72 and developing a high throughput screen to more effectively study 3E8.scFv.
Keywords: Immunhistochemistry, Cancer, High Throughput