Poster abstracts
Poster number 61 submitted by Hui Li
Phi29 motor pRNA three-way junction-based RNA nanoparticles are resistant to I-125 and Cs-131 radiation
Hui Li (College of Pharmacy/Division of Pharmaceutics and Pharmaceutical Chemistry; College of Medicine/Department of Physiology & Cell Biology/Dorothy M. Davis Heart and Lung Research Institute; OSU), Sijin Guo, Fengmei Pi, Dan Shu (College of Pharmacy/Division of Pharmaceutics and Pharmaceutical Chemistry; College of Medicine/Department of Physiology & Cell Biology/Dorothy M. Davis Heart and Lung Research Institute; OSU), Piotr G. Rychahou, B. Mark Evers (Department of Surgery; Markey Cancer Center; University of Kentucky), Zheng Cui (College of Pharmacy; University of Kentucky), Wei Luo (Department of Radiation Medicine; Markey Cancer Center; University of Kentucky), Peixuan Guo (College of Pharmacy/Division of Pharmaceutics and Pharmaceutical Chemistry; College of Medicine/Department of Physiology & Cell Biology/Dorothy M. Davis Heart and Lung Research Institute; OSU)
Abstract:
Radiation reagents that specifically target tumors are in high demand for the treatment of cancer. The emerging field of RNA nanotechnology might provide new opportunities for targeted radiation therapy. Here we investigates whether chemically modified RNA nanoparticles derived from pRNA Three-Way Junction (3WJ) of phi29 DNA-packaging motor are resistant to potent I-125 and Cs-131 radiation with therapeutic doses, which is a required perquisite for utilizing these RNA nanoparticles as carriers for targeted radiation therapy. pRNA 3WJ nanoparticles were constructed and characterized and the stability of these nanoparticles under I-125 and Cs-131 irradiation with clinically relevant doses was examined. RNA nanoparticles derived from the pRNA 3WJ targeted tumors specifically and they were stable under irradiation of I-125 and Cs-131 with clinically relevant doses ranging from 1 Gy to 90 Gy over a significantly long time up to 20 days, while control plasmid DNA was damaged at 20 Gy or higher. Therefore, RNA 3WJ nanoparticles have the potential to carry I-125 or Cs-131 for targeted radiation therapy.
References:
1. Guo P. The emerging field of RNA nanotechnology. Nature Nanotechnology. 2010. 5:833-42.
2. Shu D, Shu Y, Haque F, Guo P. Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics. Nature Nanotechnology. 2011. 6:658-67.
3. Li H, Rychahou PG, Cui Z, Pi F, Evers BM, Shu D, Guo P, Luo W. RNA Nanoparticles Derived from Three-Way Junction of Phi29 Motor pRNA are Resistant to I-125 and Cs-131 Radiation. Accepted. Nucleic Acid Therapeutics. 2015. 25:188-97.
Keywords: RNA Nanoparticles, RNA Nanotechnology, Radiation