Poster abstracts
Poster number 58 submitted by Zhengyi Zhao
Sequential Action of Asymmetrical Hexameric ATPase In DsDNA Translocation Biomotor
Zhengyi Zhao (The Ohio State University), Hui Zhang (The Ohio State University), Chad Schwartz (The Ohio State University), Peixuan Guo (The Ohio State University)
Abstract:
Traditionally, biomotors are classified into two categories: linear and rotation motors. Recently, we’ve discovered a third type of motors with revolution motion without rotation1-3. Subsequent studies lead to the finding that revolution motors are widespread among many organisms including bacteria, virus, and possibly eukaryote cells 4-6. Nature has evolved an elegant revolution motor that renders dsDNA void of coiling and tangling during the translocation of the lengthy chromosome. Here, we will focus on the utilization of biophysical, biochemical, nanotechnological, and single molecule optical approaches to elucidate the sequential action of the motor ATPase during the one-way revolution motion. We will present the mechanisms of the sequential action and subunit coordination of the hexameric ATPase, including the mechanisms of complex formation, inter-subunit communication, and changes of entropy and conformation. ATP binding induced conformation and an entropy alterations in ATPase to generate high affinity toward dsDNA; ATP hydrolysis triggered another conformational and entropic change in ATPase to a low affinity for DNA, pushing the dsDNA toward an adjacent ATP-bound subunit. The sequential pulling and pushing of the dsDNA by ATPase enabled the continuation of motor motion. The finding of asymmetrical hexameric organization is supported by structural evidences of many other biological motor systems.
References:
1. Schwartz et al. & Guo. Virology 2013, 443:28.
2. Zhao et al. & Guo. ACS Nano 2013, 7:4082.
3. Guo et al. Virology 2013, 446:133.
4. De-Donatis et al. & Guo. Cell Biosci 2014, 4:30.
5. Guo et al. Biotechnology Advances 2014, 32:853.
6. Guo et al. Microbiology and Molecular Biology Reviews 2016, 80:161.
Keywords: biomotor, subunit coordination, sequential action